Analysis Of Chronic Nicotine Administration On Carbohydrate Metabolism In The Brain Tissue Of Male Albino Rat With Reference To Aging

The present study is aimed at investigating the effect of nicotine administration on carbohydrate metabolic enzymes in the brain tissue of male albino rat. In vitro studies showed that Red grape (Vitis vinifera) has significant antioxidant activity, antimicrobial, anti-inflammatory action. Nicotine induces oxidative stress both in vivo and in vitro that causes a peroxidant/antioxidant imbalance in tissues. Pathogen free, Wistar strain male albino rats were used in the present study, Age matched rats were be divided into 4 groups of six in each group and treated as follows: Group I. Normal Control (NC) (Control rats received 0.9% saline). Group II. Nicotine treated (Nt) (at a dose of 0.6 mg/ kg body weight by subcutaneous injection for a period of 2 months). Group III. Red grape extract treated (RGEt). (Red grape extract treatment at a doses of 50 mg/ kg body weight via orogastric tube for a period of 2 months). Group IV. Nicotine treated + Red grape extract treated (Nt+RGEt) (The forth group of rats were received the nicotine + red grape extract as followed by the second and third group). The animals were sacrificed after 24 hrs after the last treatment by cervical dislocation and isolated the brain tissue such as the activities of the levels of Total Corbohydrates, Glycogen and Total free amino acids, were significantly decreased in nicotine treated rats in the brain tissue and enhance was observed in the combination treatment (Nt+RGEt), but Red grape extract treatment at a dose of 50 mg/kg body weight found to be more effective. This results stating that red grape extract treated rats were beneficial, especially for the nicotine subjects to improve the carbohydrate metabolic profile.